Erythrocyte band 3 profile in falciparum malaria and sickle cell disease:  a comparative cross-sectional study.

Ashima Mishra; Prafulla Kumar Bariha; Kshetramohan Tudu; Dukhia Murmu; Manoj Kumar Mohapatra

doi:10.51168/8p4xmy48

Autori

Ashima Mishra Senior Resident, Department of Medicine, V.S.S. Institute of Medical Sciences and Research (VIMSAR), Burla, Odisha, India. SUM Hospital, Bhubaneswar, Odisha, India
Prafulla Kumar Bariha Associate Professor, Department of Medicine, VSS Institute of Medical Science and Research, Burla, Sambalpur, Odisha, India
Kshetramohan Tudu Assistant Professor, V Department of Medicine, S.S. Institute of Medical Sciences and Research (VIMSAR), Burla, Odisha, India. 768017, SUM Hospital, Bhubaneswar, Odisha, India
Dukhia Murmu Senior Resident, Department of Medicine, V.S.S. Institute of Medical Sciences and Research (VIMSAR), Burla, Odisha, India. SUM Hospital, Bhubaneswar, Odisha, India
Manoj Kumar Mohapatra Retd. Professor Medicine, Department of Medicine, V.S.S. Institute of Medical sciences and Research (VIMSAR), Burla, Odisha, India. SUM Hospital, Bhubaneswar, Odisha, India

DOI:

https://doi.org/10.51168/8p4xmy48

Cuvinte cheie:

Band 3 protein, Falciparum malaria, Sickle cell disease, Erythrocyte membrane, Cytoadherence

Rezumat

Background

Band 3 is a major erythrocyte membrane protein involved in membrane structure, parasite invasion, cytoadherence, and sequestration. Alterations in Band 3 may contribute to the pathophysiology of falciparum malaria and sickle cell disease. This study evaluated the Band 3 profile in these conditions.

Methods

This single-center comparative cross-sectional study included patients with uncomplicated falciparum malaria, severe falciparum malaria, sickle cell disease, and healthy controls. Malaria was diagnosed by peripheral blood smear and/or rapid diagnostic testing, while sickle cell disease was confirmed by high-performance liquid chromatography. Erythrocyte membrane Band 3 was analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and densitometry. Band 3 fragmentation was assessed as high-molecular-weight aggregates, Band 3 monomer, and proteolytic fragments.

Results

Among 71 participants, 19 had uncomplicated malaria, 22 had severe malaria, 20 had sickle cell disease, and 10 were healthy controls. Relative to controls, Band 3 densitometric values were 139% in uncomplicated malaria, 138% in severe malaria, and 171% in sickle cell disease. High-molecular-weight aggregates were increased in severe malaria and sickle cell disease, whereas proteolytic fragments were reduced. Band 3 density did not differ significantly between uncomplicated and severe malaria.

Conclusion

Falciparum malaria and sickle cell disease were associated with quantitative and structural alterations in erythrocyte Band 3.

Recommendation

Larger multicenter and longitudinal studies with molecular and functional investigations are recommended to validate these findings and determine the potential diagnostic and prognostic significance of Band 3 alterations.

Biografii autori

Ashima Mishra, Senior Resident, Department of Medicine, V.S.S. Institute of Medical Sciences and Research (VIMSAR), Burla, Odisha, India. SUM Hospital, Bhubaneswar, Odisha, India

is a Senior Resident in the Department of Medicine at Veer Surendra Sai Institute of Medical Sciences and Research (VIMSAR), Burla, Odisha, India. Her research interests include tropical medicine, infectious diseases, hematology, and erythrocyte membrane biology, with a special focus on malaria and sickle cell disease.
Prafulla Kumar Bariha, Associate Professor, Department of Medicine, VSS Institute of Medical Science and Research, Burla, Sambalpur, Odisha, India

is an Associate Professor in the Department of Medicine at VIMSAR, Burla, Odisha. His academic interests include internal medicine, clinical research, and tropical infectious diseases, and he has contributed extensively to medical education and patient care.
Kshetramohan Tudu, Assistant Professor, V Department of Medicine, S.S. Institute of Medical Sciences and Research (VIMSAR), Burla, Odisha, India. 768017, SUM Hospital, Bhubaneswar, Odisha, India

is an Assistant Professor in the Department of Medicine at VIMSAR, Burla, Odisha. His research focuses on clinical medicine, hematological disorders, and evidence-based management of infectious diseases.
Dukhia Murmu, Senior Resident, Department of Medicine, V.S.S. Institute of Medical Sciences and Research (VIMSAR), Burla, Odisha, India. SUM Hospital, Bhubaneswar, Odisha, India

is a Senior Resident in the Department of Medicine at VIMSAR, Burla, Odisha. His areas of interest include internal medicine, clinical research, and management of infectious and hematological disorders.
Manoj Kumar Mohapatra, Retd. Professor Medicine, Department of Medicine, V.S.S. Institute of Medical sciences and Research (VIMSAR), Burla, Odisha, India. SUM Hospital, Bhubaneswar, Odisha, India

is a retired Professor of Medicine from VIMSAR, Burla, Odisha. He has extensive experience in tropical medicine, malaria, hematology, and clinical research, and has authored numerous scientific publications in these fields. His research has significantly contributed to understanding malaria pathogenesis and sickle cell disease in endemic regions.

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